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Fundamentals of Biotechnology

Module name (EN): Fundamentals of Biotechnology
Degree programme: Mechanical and Process Engineering, Bachelor, ASPO 01.10.2019
Module code: MAB_19_V_3.08.GBT
SAP-Submodule-No.: P241-0254
Hours per semester week / Teaching method: 4V (4 hours per week)
ECTS credits: 5
Semester: 3
Mandatory course: yes
Language of instruction:
Written exam 180 min.

[updated 05.11.2020]
Applicability / Curricular relevance:
MAB_19_V_3.08.GBT (P241-0254) Mechanical and Process Engineering, Bachelor, ASPO 01.10.2019, semester 3, mandatory course, Specialization Process Engineering
60 class hours (= 45 clock hours) over a 15-week period.
The total student study time is 150 hours (equivalent to 5 ECTS credits).
There are therefore 105 hours available for class preparation and follow-up work and exam preparation.
Recommended prerequisites (modules):
MAB_19_A_1.07.ENB Engineering Basics

[updated 18.02.2020]
Recommended as prerequisite for:
MAB_19_V_4.08.BUV Environmental and Bioprocess Engineering (with Lab Course)

[updated 18.02.2020]
Module coordinator:
Prof. Dr. Matthias Brunner
Lecturer: Prof. Dr. Matthias Brunner

[updated 28.05.2018]
Learning outcomes:
After successfully completing this course, students will be able to:
- explain the building blocks of living organisms and their function,
- map the structure of cells and their functional carriers,
- explain the potential of microorganisms and their possible uses,
- demonstrate methods for handling, preventing and mass producing microorganisms,
- describe and apply measurement and detection methods. The will have an overview of organic chemistry, biochemistry and everyday chemistry. Students will be able to explain the building blocks of living organisms and their respective functions. They will be able to explain the structure of cells and their functional carriers
They will be able to explain the potential of microorganisms, their ecology, their dangers and their possible uses.
Students will be able to explain and apply essential methods for handling, preventing and mass producing microorganisms, as well as essential measurement and detection methods.

[updated 05.11.2020]
Module content:
Hydrocarbons, alkanes, alkenes, ethers, esters, functional groups, aromatics, heterocycles, sugars, carbohydrates, carboxylic acids, pH, buffer systems, calcium carbonate balance, fats, soaps, cell structure, eukaryotes, prokaryotes, organelle evolution, amino acids, proteins, membrane proteins, enzymes, enzyme kinetics, nucleic acids, RNA, DNA,
Methods: paper chromatography, GC, HPLC, DC, gel ectophoresis, photometry, ion exchangers
Characteristic measurement technology: Gravimetry, titrimetry, potentiometry, chromatography, ampherometric photometry, enzyme test; Sterile technique: preparing culture media, buffer systems, pouring agar plates, dilution smears, enrichment culture, pure culture; Microbiological tests and working methods: disk diffusion test, zone of inhibition tests, viable bacteria count, sterile filter technology, microscopy, strain maintenance;
Handling microorganisms, preventing microbial growth, introduction to hygiene, enrichment conditions, pure cultures, culture media, culture conditions, culture methods, viable bacteria count, sterility control, streaking, disk diffusion test, antibiotics, selective energy conversion of organisms, metabolism types chemoorganoheterotrophy: glycolysis, citric acid cycle, respiratory chain, fermentation, anaerobic respiration, chemolithoautotrophy, phototrophy; Introduction to microbial ecology, ecology in lakes, seas, rivers, soils,
hygiene, waterborn and foodborn deseases, food preservation; Introduction to food biotechnology: beer, wine, cheese etc...

[updated 05.11.2020]
Teaching methods/Media:
Lecture with blackboard; copies of the slides used in the lecture

[updated 05.11.2020]
Recommended or required reading:
Brock Biology of Microorganisms, Prentice Hall
Forst et al.: Chemie für Ingenieure
Löwe: Biochemie, Benke

[updated 05.11.2020]
[Thu Sep 29 12:13:28 CEST 2022, CKEY=mgdbb, BKEY=m2, CID=MAB_19_V_3.08.GBT, LANGUAGE=en, DATE=29.09.2022]